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To investigate the antibacterial effects of PAS | IDR
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Introduction
Tuberculosis (TB) remains a significant global health concern, despite recent advancements in its management.1 Standard TB treatment respond in only 80% of patients, well below the World Health Organisation’s target of 90%.2 Multidrug-resistant TB (MDR-TB) worsens when Mtb develops resistance to anti-TB drugs.3 As a result, cure rates for MDR-TB and extensively drug-resistant tuberculosis have fallen by 50% and 30%.4 MDR-TB strains, which resist conventional anti-TB drugs, pose a challenge due to prolonged treatment periods and severe side effects, compounded by patient non-compliance leading to further drug resistance.5
Tracheobronchial tuberculosis, affecting a quarter of patients, presents additional complexities. Unlike pulmonary TB, it often requires extended treatment, sometimes necessitating local administration due to fibroplasia and caseous necrosis impeding drug penetration.6 Even with appropriate medication, tracheobronchial TB can cause irreversible narrowing, often requiring surgical intervention, imposing substantial physical and financial burdens on patients.7
In this context, exploring alternative treatment modalities is crucial. Plasma, with its potent bactericidal properties driven by reactive oxygen species (ROS), emerges as a promising avenue.8,9 At present, there are few studies on the inactivation effect of plasma on TB, and most of them focus on the gaseous state of plasma.10,11 Lee C12 proved that OH radicals in a Nonthermal Plasma Jet (NTPJ) were used to inactivate mycobacteria in aqueous solution, which can inactivate nonpathogenic Mycobacterium smegmatis and pathogenic Mtb H37Rv. Bar W10 proved that the low-temperature hydrogen peroxide gas plasma had a bactericidal effect on the bronchoscope infected by Mtb. Daeschlein G’11 experiments showed that Atmospheric Plasma Jet (APPJ) and Dielectric Barrier Discharge (DBD) Plasma had high inactivating effects on 8 different Mtb strains. It has also been proved that PAS has strong sterilization activity against Shewanella putrefaciae and methicillin-resistant Staphylococcus aureus (MRSA).13,14 Unlike traditional drug-based approaches, plasma-based therapy offers several advantages, including broader clinical applicability, particularly in irrigating sites affected by TB without contributing to drug resistance.
This study evaluates the in vitro antibacterial activity of PAS against Mtb, presenting a novel therapeutic approach for TB treatment. By harnessing the power of plasma, this method offers a potential solution to the challenges posed by drug-resistant TB strains and the limitations of conventional treatments.
Material and Methods
Plasma Device and Preparation of PAS
PAS is produced by the plasma effluent gas enriched with high valence NOx, which is generated by the plasma discharge configuration, similar to our previous studies.15 The setup comprises two discharge reactors: a sliding electric arc and a coaxial DBD. The sliding electric arc reactor consists of two stainless steel blades positioned opposite each other (minimum distance = 2 mm, blade length = 35 mm) and an air nozzle with an inner diameter of 1 mm. The blade electrodes are powered by a high-voltage transformer (TH5-NT-1530, Sinolift, Shanghai, China) at approximately 20 W, generating NOx−dominated plasma effluent gas. This is because the high temperature of the sliding electric arc completely suppresses the generation of O3, with NO and NO2 becoming the primary gaseous reactive species produced. The coaxial DBD reactor involves a quartz tube (outer diameter = 18 mm, thickness = 1 mm) with a ground electrode covering the outer wall (length of 150 mm). A high-voltage electrode is placed at the axis of the quartz tube with a 1mm gap between the high-voltage electrode and the inner wall of the quartz tube. The DBD is powered by a high-voltage power supply (CTP-2000K, Suman, Nanjing, China) with a discharge power of 15 W. In the coaxial DBD reactor, the dissociation energy threshold of O2 is higher than that of N2, making O3 the primary product of the coaxial DBD reactor. Air is introduced separately into the sliding electric arc reactor and coaxial DBD reactor with airflow rates of 1 SLM and 5 SLM, respectively. The two paths of plasma effluent gas are mixed in the gas tube, and the mixed plasma gas is introduced into 100 mL of normal saline for a 15minutes treatment to produce PAS, as shown in Figure 1.
Figure 1 The Machine of the production of plasma-activated saline. |
Measurement of Gaseous and Aqueous Plasma Reactive Species
To investigate the gas-phase characteristics of the plasma effluent gas, Fourier-transform infrared (FTIR) spectroscopy is conducted. The mixed plasma effluent gas is introduced into a gas cell with an optical path of 2.4 m, which is integrated with an FTIR spectrometer (Tensor II, Bruker, Billerica, Massachusetts, USA). The absorption cross-section data of each gaseous reactive species is taken from the HITRAN database16. For the detection of aqueous reactive species, TEMPONE-H (1-hydroxy-2, 2,6, 6-tetramethyl-4-oxo-piperidine, Enzo, final concentration = 1mM) is used as a spin trap for O2− and ONOO− in PAS, and their concentrations are measured by an electron spin resonance spectrometer (EMXplus, Bruker, Billerica, Massachusetts, USA). The concentration of NO in the aqueous solution is quantized by another spin trap MGD (N-methyl-D-glucaminedithio-carbamate, Dojindo, final concentration = 10 mM). For the long-lived species including H2O2, NO2−, and NO3−, their concentrations are measured by using a microplate reader (Thermo, Varioskan Flash) with the hydrogen peroxide assay kit (Beyotime, Shanghai, China) for H2O2 and the nitrate/nitrite colorimetric assay kit (Beyotime, Shanghai, China) for NO2− and NO3−. The physicochemical parameters of PAS, including pH and oxidation-reduction potential (ORP), were measured using a pH/ORP meter (S210, Mettler Toledo, Zurich, Switzerland). The measurements of the concentration of aqueous reactive species and the physicochemical properties of PAS have been done three times repeatedly.
Strains of Mtb
We collected 6 strains of Mtb, which were isolated and identified by the Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, numbered 5158, 7281, 7454, 9545 and the virulent Mtb strain H37Rv, with the informed consent of the patients. Mtb strains 72819545 and H37Rv were tested for growth assay. Among the 4 strains included in the drug sensitivity test, strain 7281 was found to be resistant to SM, INH, RIF and EMB, while strains 5158, 9545, and 7454 were resistant to SM, INH, and RIF, but sensitive to EMB. This study was approved by the Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment. The experiments we conducted were under Biosafety Level 3 conditions.
Bacterial Suspension Preparation
To prepare the bacterial suspension, NS was drawn into a sterile tube using a sterile pipette. Fresh colonies from a 2-week-old culture were then collected using an inoculating loop and transferred into the sterile tube. The tube was tightly sealed, and the contents were mixed by shaking at a concentration of 1 McFarland standard (1 mg/mL = 3×108 CFU/mL) using an ultrasonic disruptor. The prepared bacterial suspension was then centrifuged at 3000 rpm for 5 minutes, and the supernatant was discarded. The pellet was resuspended in the appropriate diluent to achieve a concentration of 1 McFarland (1 mg/mL) bacterial suspension. Using a micropipette, 50 μL of the 1 McFarland bacterial suspension was aspirated and added to 5000 μL of the corresponding diluent to obtain a concentration of 106. Using a pipette to absorb 106 CFU/mL bacterial solution 50 μL and add 5000 μL diluent to obtain 104 CFU/mL bacterial suspension.
Growth Assay
The suspensions of each strain were divided into three groups: NS control group, PAS-treated group, and HCl-treated group, with three replicates per group. The procedures are as follows:
NS Control Group: Dilute the bacterial suspension to a concentration of 104 CFU/mL based on the 1 McFarland standard. Using a pipette, transfer 100 μL of the diluted bacterial suspension and evenly inoculate it onto the surface of the roche culture tube (Baso, Zhuhai, China), ensuring the bacterial suspension is evenly dispersed on the agar slope. Take 500 μL of the diluted bacterial suspension and inoculate it into the prepared BD culture medium (Becton Dickinson, Shanghai, China).
PAS Treatment Group: Take the purified 1 McFarland bacterial suspension and centrifuge it to remove the supernatant. Add 2 mL of PAS to the bacterial pellet. After 30 minutes, follow the same procedure as the NS control group for inoculation.
HCl Treatment Group: Adjust the pH of the HCl with NS to match that of the PAS. Follow the same procedure as the PAS control group for inoculation.
Since Mtb grows slowly, it usually takes 7 days to observe visible colonies on the culture medium, and sufficient colonies are obtained after 14 days. Therefore, the bacterial colony counts will be recorded on day 14, 20, 26, and 31. The selection of these time points is based on ensuring a sufficiently long duration between them to observe significant changes.
Drug Sensitivity Test
NS control group: Adjust the clinical strains 5158, 7281, 7454, and 9545 to a concentration of 1 McFarland. Pipette 20 μL of the adjusted bacterial suspension into 2 mL of NS solution (10−2 dilution). Pipette 20 μL of the 10−2 bacterial suspension into 2 mL of NS solution (10−4 dilution). Inoculate the drug-containing roche culture tube with 10 μL using an inoculation loop.
PAS treatment group: Adjust the clinical strains 5158, 5943, 7281, 7454, and 9545 to a concentration of 1 McFarland. Pipette 20 μL of the adjusted bacterial suspension into 2 mL of PAS (10−2). Pipette 20 μL of the 10−2 bacterial suspension into 2 mL of NS solution (10−4). Allow the suspensions to stand for 30 minutes. Inoculate the drug-containing roche culture tube with 10 μL using an inoculation loop.
HCl treatment group: Follow the same procedure as the PAS treatment group, but replace the PAS with HCl.
The bacterial colonies of Mtb will be counted on the 20th, 26th, and 31st days in the drug-containing roche culture tube.
Bacterial Colony Counts
Report of bacterial growth on drug-containing and control roche culture tube: Mtb culture negative (-): No colony growth observed on the slant. Mtb culture positive (+): Colony growth covering 1/4 of the slant area. Mtb culture positive (++): Colony growth covering 1/2 of the slant area. Mtb culture positive (+++): Colony growth covering 3/4 of the slant area. Mtb culture positive (++++): Colony growth covering the entire slant. As shown in the Figure 2, from left to right, different degrees of growth of Mtb are represented.
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Figure 2 The growth of Mtb in different degrees: (A–D) respectively represents Mycobacterium tuberculosis culture positive (+), (++), (+++) and (++++). |
Statistical Analysis
Statistical analysis was performed using SPSS 26.0 (Chicago, IL, USA). The non-parametric Kruskal–Wallis test was used to compare differences among multiple groups. If the overall test did not detect significant differences between groups, pairwise comparisons were not conducted. Statistical significance was established at a P-value
Results
PAS Worked Due to the Reactive Species Therein
Given that PAS is produced by introducing plasma effluent gas into a saline solution, it is necessary to investigate the gaseous reactive species in the plasma effluent gas. The FTIR absorption spectra of the mixed plasma effluent gas generated by the hybrid plasma discharge configuration are illustrated in Figure 3. The coaxial DBD reactor and sliding electric arc reactor provide mainly O3 and low valence NOx (including NO and NO2), respectively. In the mixed gas, O3 oxidizes the low valence NOx to form high valence NOx (including N2O5 and NO3), where NO is first oxidized by O3 to NO2, and then NO2 is further oxidized by O3 to NO3, and finally NO2 and NO3 react to produce N2O5. N2O is also present in the mixed plasma effluent gas, but it is an inert gaseous component that is stable and hardly reacts.
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Figure 3 FTIR absorption spectra of the mixed gas from DBD and gliding arc discharge. Abbreviations: FTIR, Fourier-transform infrared; DBD, Dielectric Barrier Discharge. |
Various aqueous reactive species are produced in PAS as a result of a series of chemical reactions between gaseous reactive species and aqueous solutions. As shown in Figure 4, the experimental results indicate that in the 100 mL of PAS after 15 min of plasma treatment, the concentrations of H2O2, NO2−, NO3−, O2−andONOO−, and NO radicals are 190.08±53.34 μM, 148.08±35.72 μM, 8564.33±523.81 μM, 26.44±6.92 μM, and 9.03±2.37 μM, respectively. In addition, the physicochemical parameters measured by the pH/ORP meter show that the pH and ORP values of the PAS are 2.01±0.11 and 639±43.62 mV, respectively.
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Figure 4 Concentrations of aqueous reactive species in plasma-activated saline. |
Growth Assay
Observation of the growth of Mtb at different time points (Day 14, 20, 26, and 31), revealed the following findings (Table 1): At all observed time points, there was a significant difference in the growth of Mtb between the NS control group and the PAS treated group. As time progressed, significant differences in the growth of Mtb were observed between the PAS-treated group and the HCl-treated group on Day 20, 26, and 31(Table S1). No significant difference in the growth of Mtb was observed between the NS group and the HCl-treated group throughout these time points. It is noteworthy that for the bacterial count in the PAS-treated group was significantly lower than that in the NS group or HCl group at the final observation time point (Day 31). Figure 5 shows the growth of TB strain 7281,9454, H37Rv in different groups on day 31st.
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Table 1 Statistic Analysis of Growth Experiment |
Drug Sensitivity Test of INH, SM and RIF
Strains 7281, 9545, 7454 and 5158 were resistant to INH, SM, and RIF. Observation of the growth of Mtb at a bacterial concentration of 10−2, revealed the following findings (Table 2): In the INH drug sensitivity test, there was no statistically significant difference in the growth of Mtb between the NS group and the HCl-treated group. The growth of Mtb in the PAS-treated group was slower compared to the other two groups (Table S2). In the SM drug sensitivity test, with the progression of time, the growth of Mtb in the PAS-treated group became progressively slower compared to the other two groups (Table S2). Similar to the INH test, there was no difference in the growth of Mtb between the NS group and the HCl-treated group (Table 2). Although no significant differences were observed in the remaining drug sensitivity test, the overall trend was that the growth of Mtb in the PAS-treated group slower than that of the NS group and the HCl-treated group as time progressed (Table S2), especially on the 31st day, as shown in Figure 6.
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Table 2 Statistic Analysis of Drug Sensitivity Experiment (Bacterial Suspension Concentration=10−2) |
Observation of the growth of Mtb at a bacterial concentration of 10−4, revealed the following findings (Table 3): In the SM drug sensitivity test, there was a difference in the growth of Mtb between the NS group and the other two groups at 31st day, but there was no difference between the PAS and HCl groups at the same time. Although no statistically significant differences were observed in the remaining drug sensitivity test, there are still the following expected results (Table S3): In the drug sensitivity test for RIF, strain 5158 exhibited colony formation in both the NS and HCl group, while no colony formation was observed in the PAS group. In the drug sensitivity test for INH, strain 7454 displayed varying degrees of growth in both the NS and HCl group, but no colony formation was observed in the PAS group. Similarly, in the drug sensitivity test for SM, strain 7281 showed varying degrees of growth in both the NS and HCl groups, but no colony formation was observed in the PAS group.
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Table 3 Statistic Analysis of Drug Sensitivity Experiment (Bacterial Suspension Concentration=10−4) |
Drug Sensitivity Test of EMB
Strain 7281 is an EMB-resistant strain. The growth of strain 7281 was observed at different bacterial concentrations, and no statistically significant differences were found (Table 4).
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Table 4 Drug Sensitivity Experiment of 7281 in EMB |
It is important to highlight that in the drug sensitivity test conducted with a bacterial concentration of 10−2, the growth of strain 7281 in the PAS group consistently exhibited slower growth compared to the NS and HCl groups, as shown in Figure 6, on day 31, the number of TB bacterial colonies visible in the PAS group was less than in the other two groups. Additionally, in the drug sensitivity test performed with a bacterial concentration of 10−4, no colony formation was observed in any of the three groups.
Strain 5158, 9545, and 7454 were sensitive to EMB. There was no difference in the growth of Mtb in different groups, whether the concentration of bacteria was 10−2 or 10−4 (Table 5). Although no Mtb growth was observed at 10−4 bacterial concentration (Table S3), the growth of Mtb in PAS treatment group was significantly less than that in NS and HCl groups, which could be observed in strains 7454 and 5158 at 10−2 bacterial concentration (Table S2, Figure 6).
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Table 5 Drug Sensitivity Experiment of 5158, 9545, and 7454 in EMB |
Discussion
Currently, TB prevention and control in China still face a very severe situation. TB is not only a serious public health problem that poses a threat to the health of the Chinese population but also a significant social issue that hinders national progress and economic development. The current challenges in TB treatment include drug resistance and the lack of effective new drugs. In recent years, the emergence of many innovative treatment methods has provided new therapeutic strategies to end tuberculosis, such as endoscopic injection of anti-tuberculosis drugs, cryotherapy, and bronchial stent implantation.17 However, these treatments may lead to drug resistance or fail to eliminate the lesions. Therefore, there is an urgent need to search for a treatment method that has fewer toxic side effects and better therapeutic outcomes.
Plasma biomedicine is an emerging interdisciplinary field in recent years, combining plasma science and technology, life sciences, clinical medicine, and other disciplines. The high valence NOx radicals in the mixed plasma effluent gas reacted with water to generate large amounts of H+ and NO3−, resulting in the acidity of PAS, which helps to ensure high bioactivity.18 Besides, the aqueous H2O2 is likely to originate from the dissolution of gaseous H2O2,15 whereas the aqueous NO radicals and NO2− have been reported to be produced from gaseous NO2.19 Most importantly, in addition to HNO3, the gaseous high valence NOx can generate short-lived aqueous reactive species such as O2− and ONOO−,20 which enables the remarkable chemical and biological reactivity of PAS. The gaseous NO3 is considered to be the most reactive among NOx and may play the most crucial role in water activation. Currently, plasma has been used for medical device sterilization,10 treatment of severe infections14 and cancer.21 The plasma can promote wound healing by promoting the production of various reaction mediators, such as H2O2, hydroxyl radical, O3, and up-regulating growth factors such as VEGF and FGF.22 Studies have shown that these mediators have a certain antibacterial effect on Staphylococcus aureus, and can improve the sensitivity of Staphylococcus aureus to antibiotics and enhance the inactivation of antibiotics.23 ROS produced by plasma can induce the apoptosis of gastric cancer cells.24
This study focuses on the liquid form of plasma and successfully produces PAS. It detects activated nitrates, nitrites, H2O2, and some activated ions, including O2−,·OH, ONOO− and other active components. The study explores the antibacterial effects of PAS on Mtb through growth assay. By comparing the growth of 3 strains of Mtb in different treatments at 4 observation time points, it was found that the bacterial colony counts of Mtb in the PAS treatment group were less than that in the NS-treated group. Furthermore, at the final observation time point, the bacterial colony counts of Mtb in PAS were less than that in the corresponding NS group and HCl group. This result preliminarily demonstrates the antibacterial effect of PAS on the growth of Mtb.
However, the results indicate no statistically significant difference between PAS and HCl treatment groups on the 14th day, suggesting that pH may affect the growth of Mtb. Pyrazinamide (PZA), a first-line drug for tuberculosis treatment, exerts its bacteriostatic effect on Mtb only in an environment with a pH below 5.5.25 This leads to a preliminary inference: The low pH of PAS may affect the growth of Mtb. Based on the subsequent test results, statistical differences were observed between PAS and HCl on days 20, 26, and 31. Excluding the influence of pH, PAS can still inhibit the growth of Mtb, possibly because ROS and other long-lived and short-lived reactive species released by PAS affect the growth of Mtb.
Next, we investigated whether PAS affected the sensitivity of drug-resistant Mtb to anti-TB drugs (INH, SM and RIF). PAS significantly enhanced the sensitivity of drug-resistant Mtb to INH and SM compared with NS in the drug-sensitivity test with a bacterial suspension concentration of 10−2. However, there was no statistical difference observed between the HCl group and the NS group. This indicates that PAS can significantly enhance the drug sensitivity of drug-resistant Mtb to INH and SM even if the pH factor is excluded. In experiments with bacterial suspension concentration of 10−4:PAS only affected the sensitivity of Mtb to SM, and did not show significant effects on other drugs. This could be attributed to the low bacterial concentration in the culture.
As the most common drug-resistant form26, there is a close connection between the mechanism of action of INH and plasma. INH enters the interior of Mtb cells through passive diffusion and is activated by catalase and peroxidase (KatG), which disrupts the growth of Mtb by affecting its internal homeostasis environment.27 Interestingly, KatG expression in Mtb is peroxide-induced.28 The activation of INH within the TB produces the same free radicals and superoxide produced by plasma. Timmins showed by Electron spin capture (EPR) that INH activated by KatG produces nitric oxide (NO).29 Studies have found that endogenous superoxide contributes to the intracellular activation of INH.30
It is worth noting that in the INH drug sensitivity test with a bacterial concentration of 10−4, strain 7454 showed varying degrees of growth in both the NS and HCl groups, but no colony formation was observed in the PAS groups. This result indicates that PAS can significantly enhance the sensitivity of INH-resistant Mtb to INH. One possible reason for this is that the reactive radicals and oxidants generated after plasma activation can enhance the expression of KatG and activate INH within the Mtb cells.27 Additionally, the strong cell-penetrating ability and low pH of plasma can influence the internal environment of Mtb, thereby affecting its normal biological processes and achieving the effect of anti-tuberculosis treatment.31
Upon observing the drug sensitivity test of EMB-resistant strain 7281, it is notable that no statistically significant differences were observed between the 10−2 and 10−4 bacterial concentration groups. In the drug sensitivity test with a bacterial concentration of 10−2, the group treated with PAS consistently displayed slower colony formation compared to the NS and HCl groups. This suggests that PAS may have altered the resistance of strain 7281 to EMB. Combined with the subsequent drug sensitivity test results of EMB-sensitive TB strains, PAS can further enhance the sensitivity of Mtb to EMB and inhibit their growth. We can speculate that PAS may inhibit the growth of both drug-resistant and drug-sensitive TB strains at the same time.
Certainly, there are limitations and shortcomings in this study. Firstly, the number of bacteria included in the experiments was relatively small, and further validation is needed using a larger sample size of Mtb. Secondly, although this study included an HCl control group to eliminate the influence of pH on Mtb, the specific role of pH in inhibiting the growth of Mtb needs further clarification. Additionally, the mechanisms by which ROS and hydroxyl radicals produced in PAS inhibit the growth of Mtb require further investigation.
In summary, the use of PAS for the treatment of tuberculosis is a highly promising strategy. The results of this study demonstrate that PAS has an impact on the growth activity of both drug-sensitive and drug-resistant strains of Mtb. In the next step, we will investigate the specific mechanisms by which PAS inhibits the growth of Mt. Our aim is to apply it in the lavage of bronchial tuberculosis, tuberculous empyema, and joint tuberculosis in the future.
Conclusion
This study demonstrates that PAS, generated by introducing plasma effluent gas into saline, contains a significant amount of active substances, including H2O2, NO2−, NO3−, O2−andONOO−. Moreover, PAS exhibits high oxidizability and presents an acidic pH state.
Furthermore, the growth experiments indicate that compared to the NS group and the HCl group, the unique physicochemical properties of PAS can significantly delay the growth of Mtb.
In drug susceptibility testing, we observed that PAS significantly inhibits the growth of Mtb, including both drug-sensitive and drug-resistant strains. It is noteworthy that PAS not only inhibits the growth of drug-sensitive strains but also significantly enhances the sensitivity of drug-resistant strains to anti-tuberculosis drugs, especially INH and SM.
In conclusion, although our study requires further validation and refinement, particularly through larger sample sizes and more comprehensive mechanistic studies, these findings suggest that PAS may become a promising novel therapeutic approach in the treatment of tuberculosis. PAS may have potential clinical applications in treating tuberculosis manifestations such as bronchial tuberculosis, tuberculous pleurisy, and joint tuberculosis.
Ethics Statement
This study was approved by the Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment. (Ethics approval number: 2023(No.8)). Our study complies with the Declaration of Helsinki.
Funding
This research was funded by Shaanxi Provincial Health Science Research Project (Grant number: 2022D043), the Science and Technology Project of Xi’an City (Grant serial number: 23YXYJ0155), the Nanjing Tianqing Research Fund Project (Grant number: HX202334) and the Institute Fund from First Affiliated Hospital of Xi’an Jiaotong University (Grant number: 2022MS-17), the Science and Technology Project of Shaanxi Province (Grant serial number: 2022SF-423).
Disclosure
The authors declare no conflicts of interest in this work.
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